Isolation and characterisation of some endophytic bacteria. Isolation and molecular identification of endophytic bacteria from. Furthermore, the isolation of endophytic bacteria from withania coagulans and olea ferruginea is novel in association to these plants. Omonrice 12 2004 95 the two hosts are given in table 1. Rhizome of ginger zingiber officinale was collected from navajyothisree karunakara guru research centre for ayurveda and siddha, uzhavoor, kottayam and was used as the source material for the isolation of endophytic bacteria. Isolation of endophytic fungi from thermopsis lanceolata and their antioxidant activity lin yang1, xiaokai zhou1, lei wang1, hongxia shi2, xiaofeng liu1, yonggang wang1 1the key lab of screening, evaluation and advanced processing of tcm and tibetan medicine, gansu educational department. Plating of triturated seed samples showed that total cultured bacterial densities were significantly different p. Isolation is done to obtain pure bacterial cultures.
Isolation of endophytic bacteria from dendrobium stems. Isolation and characterization of endophytic colonizing bacteria. Many of the cultivated endophytic bacteria were shown to produce the plant growth hormone indoleacetic acid, and this trait was more. To our knowledge, the present work is the first report on the isolation, identification and enzymatic characterization of endophytic microorganisms from r. B plating and isolation of bacteria on tryptic soy agar tsa, luria broth agar lb, reasoners 2a agar r2a, nutrient agar n and potato dextrose agar. Based on the culturedependent method, a total of 165 cultivable endophytic bacteria isolates were isolated from the sterilized dendrobium. These include both culturable and unculturable bacteria belonging to acidobacteria, actinobacteria. Isolation and characterization of endophytic colonizing. Isolation of endophytic bacteria from rambutan fruit in sap produces nine isolates which were. Isolation of antagonistic endophytes from banana roots. Isolation, characterization, endophytic bacteria, antibacterial, mangrove. Diversity of endophytic bacteria from eucalyptus species. Isolation and molecular identification of endophytic bacteria from rambutan fruits nephelium. Abstract endophytic bacteriareside with in plant hosts without causing disease.
The isolation of the endophytic bacteria was conducted within 48 h of the fieldwork. Medium and main reagents endophytic bacteria were isolated and cultured in na culture media containing 3 g beef extract, 5 g peptone, 5 g nacl, 18 g agar, and ml water with ph 7. Downloaded from the university of groningenumcg research database pure. Diversity of cultureindependent bacteria and antimicrobial activity of. Screening, isolation, biochemical and plant growth promoting characterization of endophytic bacteria. Isolation and molecular identification of endophytic. Isolation and characterization of plant growth promoting. Isolation and identification of endophytic fungi from. Pdf isolation and characterization of endophytic bacteria isolated. Isolation of endophytic fungi from zygophyllum mandavillei. Endophytic bacteria in agricultural crops article pdf available in canadian journal of microbiology 4310. This endophytic bacterial community may be affected by crop management such as the use of chemical compounds. Isolation and screening of p solubilising endophytic.
Isolation and genomic characterization of six endophytic. Isolation, identification and screening of endophytic. Pdf endophytic bacteria and their potential applications. The present work was aimed to isolate the endophytic bacteria from different medicinal plants such as aloe barbadensis,adhatoda vasica,phyllanthus amarus. Putative endophytic bacterial strains were defined as isolates that were obtained from surfacesterilized plants, displayed differentiable colony morphologies, and were recovered from the initial survey of agronomic crops and prairie grasses. Isolation of endophytic bacteria the surfacedisinfected plant organs roots, stems, and leaves were removed with a sterilized razor blade, and the organs were cut into pieces 1 3 mmlong, which were placed on tsa plates. Isolation and characterization of endophytic bacteria in soybean glycine sp. Screening, isolation, biochemical and plant growth promoting. This was the first study to evaluate their cytotoxic and antitumour activities against brine shrimp and five types of tumour cells, respectively. Isolation and characterization of bacterial endophytes of. Isolation and characterization of endophytic bacteria from. Pdf endophytic bacteria are ubiquitous in most plant species, residing latently or actively colonizing plant tissues locally as well.
This endophytic bacterial community may be affected by crop management such as the use. Isolation, identification and characterisation of endophytic. Isolation of antagonistic endophytes from banana roots against meloidogyne javanica and their effects on soil. Isolation and pcr amplification of the v3v4 16s rdna region of endophytic bacterial isolates from saccharum sp a saccharum sp from jamaica used for the isolation of endophytic bacteria. View endophytic bacteria research papers on academia. Isolation and characterization of antibacterial endophytic bacteria. Pdf endophytic bacterial isolates were isolated from roots, nodules, leaves and stems of faba bean vicia faba, pea pisum. Nov 01, 2010 endophytic bacteria are harmless in most plant species.
Endophytic fungi from the chinese medicinal plant actinidia macrosperma were isolated and identified for the first time. Biocontrol agents, endophytic bacteria, plantgrowthpromoting traits. Isolation and characterization of endophytic bacteria from wild and traditionally cultivated rice varieties. A read is counted each time someone views a publication summary such as the title, abstract, and list of authors, clicks on a figure, or views or downloads the fulltext. Isolation and characterization of antibacterial compound from. Vegab and soroush parsaa aentomology, international center for tropical agriculture ciat, cali, colombia. A novel method to scale up fungal endophyte isolations. The plates were examined for growth after incubation at 288c for 3 days. Isolation and characterization of endophytic bacteria.
Berg and hallmann6 confirmed that a significant portion of the indigenous endophytic bacteria in plant roots have antagonistic potential toward fungal pathogens, but little is known about the fluctuation and structure of the aeb population. Isolation of endophytic bacteria from experimentally inoculated plants. May 30, 2017 isolation and identification of endophytic bacteria with plant growth promoting and biocontrol potential from oak trees. A total of 7, 422 reads and 655 otus were obtained after clustering at a 97%. Yan x, wang z, mei y, wang l, wang x, xu q, peng s, zhou y and wei c 2018 isolation, diversity, and growthpromoting activities of endophytic bacteria from tea cultivars of zijuan and yunkang10. Abid ullah 1, hafsa mushtaq 2, usman ali 1, hakim 1, ehsan ali 3, samavia mubeen2. Bacterial endophytes in agricultural crops canadian journal. Zinniel dk, lambrecht p, harris nb, feng z, kuczmarski d, higley p, ishimaru ca, arunakumari a, barletta rg, vidaver ak 2002 isolation and characterisation of endophytic colonising bacteria from agronomic crops and prairie plants. Isolation and characterization of endophytic bacteria from plectranthus tenuiflorus medicinal plant in saudi arabia desert and their antimicrobial activities. For instance, application of glyphosate herbicide is common mainly due to the use of glyphosateresistant transgenic plants.
Alkalibacillus was used as an outgroup which derived from the same family with bacillus. Isolation and identification of indigenous endophytic bacteria. Isolation and enzyme bioprospection of endophytic bacteria. The fungus cladosporium cladosporioides was the predominant isolated fungus with colonization and dominance frequencies percentage 12. The isolation, identification and screening of endophytic bacteria for the plant growth regulators like cytokinin are needed to get the leads for their applications in agriculture sector. Bioactivities of bacterial endophytes isolated from leaf tissues of. Frontiers isolation, diversity, and growthpromoting. Isolation of putative endophytic diazotropic bacteria for p solubilisation endophytic bacteria were isolated from different parts of selected rice plant such as root and employing standard microbiological methods.
Five different taxa were represented by 11 isolates, and six isolates were. Mundt and hinkle, 1976 more than 200 bacterial genera from 16 phyla have been reported as endophytes. University of groningen bacterial endophytes of rice hardoim. Jun 21, 2012 chemical isolation and characterization of active compound. Endophytic bacteria were found in both the roots and the stems of sugarcane plants, with a significantly higher density in the roots. Endophytic bacteria are ubiquitous in most plant species influencing the host fitness by disease suppression, contaminant degradation, and plant growth promotion. Diversity of cultivated endophytic bacteria from sugarcane. Isolation procedures for endophytic microorganisms. Owing to these characters, the representative isolated bacteria are suggested to be used in biofertilizer and biopesticides. Endophytic bacteria normally live on intercellular spaces that contain carbohydrates, amino acids, and high amounts of inorganic nutrients bacon and. Isolation of potential antimicrobial metabolites from. In addition, speculative conclusions are raised on some points to stimulate thought and research on endophytic bacteria. The plant material used in this study was the above ground parts of chelidonium majus plants collected at the flowering stage between isolation and characterization of bacterial endophytes of chelidonium.
Isolation and identification of endophytic bacteria with. Laboratory manual of standardized methods for antimicrobial sensitivity tests for bacteria isolated from aquatic animals and environment. Jul 28, 2014 however, studies focusing on the isolation and characterization of endophytic bacteria from native plant specimens from the brazilian mangrove biome are rare. An array of 76 endophytic bacterial isolates obtained. We successfully isolated endophytic bacteria from six out of 10 eucalyptus species and studied hybrids. Endophytic bacteria with plant growth promotion and. Endophytic bacteria colonize a large number of plants, which include plant growthpromoting bacteria. Ruan y, tao c, chao y, li r and shen q 2017 isolation of antagonistic endophytes from banana roots against meloidogyne javanica and their effects on soil nematode.
All solvents used for extractions, isolation and purification were of hplc grade. Since the first reliable reports about the isolation of endophytic bacteria from surfacesterilized plants samish et al. From 51 sugarcane samples of 7 districts sites ben cau. Isolation, identification and screening of endophytic bacteria antagonistic to biofilm formers hira muzzamal, rabbia sarwar, imran sajid and shahida hasnain department of microbiology and molecular genetics, university of the punjab, quaideazam campus, lahore 54590, pakistan abstract. Endophytic bacteria form associations with plants, at least in one phase in their life cycle. Following incubation, cell free culture medium was concentrated under vacuum to reduce the volume to approximately 800 ml. Bacteria isolation, colony characteristic and microscopic examination the endophytic bacteria developed in the pellicles of semi solid in lgi medium as the previous results of weber et al. Jul 17, 2019 diversity of endophytic bacteria based on illuminabased analysis quality control and analysis of sequencing data. Pdf isolation and identification of fungal endophytes. Screening of endophytic bacteria isolated from leaves of.